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Rout, G. R.

Establishing Taxonomic Identity and Selecting Genetically Diverse Populations for Conservation of Threatened Plants Using Molecular Markers

Abstract Views :289 | PDF Views:93

Authors

N. K. Chrungoo ¹, G. R. Rout ², S. P. Balasubramani ³, P. E. Rajasekharan ⁴, K. Haridasan ³, B. R. P. Rao ⁵, R. Manjunath ³, G. Nagduwar ³, P. Venkatasubramanian ³, A. Nongbet ¹, M. Hynniewta ¹, D. Swain ², S. Salamma ⁵, K. Souravi ⁴, S. N. Jena ⁶, S. K. Barik ¹

Affiliations
1 Department of Botany, North-Eastern Hill University, Shillong 793 022, IN
2 Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar 751 003, IN
3 Trans-Disciplinary University, Foundation for Revitalisation of Local Health Traditions, 74/2, Jarakabanade Kaval, Attur PO, Yelahanka, Bengaluru 560 106, IN
4 Division of Plant Genetic Resources, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bengaluru 560 089, IN
5 Department of Botany, Biodiversity Conservation Division, Sri Krishnadevaraya University, Anantapur 515 003, IN
6 CSIR-National Botanical Research Institute, Lucknow 226 001, IN

Source

Current Science, Vol 114, No 03 (2018), Pagination: 539-553

Abstract

The extent of genetic diversity within a species is an important determinant of successful adaptation to adverse environmental conditions. Assessment of extent of genetic diversity/variability is also important to monitor genetic erosion within a species. In threatened plant species, genetic diversity assessment helps in selection of genetically diverse populations to enrich the genetically impoverished populations, thus minimizing the probability of genetic drift. Confirming taxonomic identity of threatened species, particularly those belonging to species complexes with dispute identity, is another essential task in the conservation of threatened species, which is best resolved through molecular approaches. The present study estimated the genetic variability within and among the populations of four threatened species, viz. Justicia beddomei (C.B. Clarke) Bennet (Acanthaceae), Embelia ribes Burm. f. (Myrsinaceae), Madhuca insignis (Radlk.) H.J. Lam (Sapotaceae) and Cycas beddomei Dyer (Cycadaceae) using Inter Simple Sequence Repeat (ISSR) and Simple Sequence Repeat (SSR) markers for selecting the genetically diverse populations. The phylogeny was analysed through ITS (nrDNA) and matK (cpDNA) sequences to confirm the species identity.

The phylogenetic analyses confirmed four distinct species of Justicia, which also revealed that J. beddomei and J. adhatoda were sister groups with a common ancestor showing rapid parallel speciation with J. gendarussa in one clade and J. betonica in another. Madhuca insignis with extremely small population in the Western Ghats (Karnataka to Kerala) might have undergone either extensive hybridization or incipient speciation. In case of Embelia species, a greater evolutionary closeness between E. subcoraceae and E. floribunda was revealed, while E. ribes had a distinct clad. Both ISSR and SSR markers distinguished various genotypes of Cycas beddomei.

Keywords

Conservation, Genetic Variability, Molecular Markers, Phylogeny, Threatened Plants.

Full Text

References

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In vitro Propagation of Some Threatened Plant Species of India

Abstract Views :237 | PDF Views:99

Authors

C. R. Deb ¹, G. R. Rout ², A. A. Mao ³, S. K. Nandi ⁴, R. K. Nilasana Singha ³, D. Vijayan ³, T. Langhu ¹, Z. P. Kikon ¹, S. Pradhan ³, Mohd Tariq ⁴, D. Swain ²

Affiliations
1 Department of Botany, Nagaland University, Lumami 798 627, IN
2 Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar 751 003, IN
3 Botanical Survey of India, Eastern Regional Centre, Shillong 793 003, IN
4 G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, Almora 263 643, IN

Source

Current Science, Vol 114, No 03 (2018), Pagination: 567-575

Abstract

To prevent extinction of threatened species, in vitro regeneration protocols for the propagation of six threatened species were standardized. The regenerated micro-shoots were ischolar_mained in nutrient medium supplemented with low concentrations of auxin. The well-developed plantlets were successfully established in field conditions, thus improving the probability of self-sustenance of the introduced populations. The success story of these six threatened species reaffirms the role of in vitro propagation in conserving plants facing the threat of extinction.

Keywords

Conservation, In vitro Regeneration, Microshoots, Threatened Species.

Full Text

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A New Growth Medium for Identification and Isolation of Pyricularia grisea From Finger Millet (Eleusine coracana L. Gaertn.)

Abstract Views :277 | PDF Views:73

Authors

K. R. Jadhao ¹, G. R. Rout ¹

Affiliations
1 Department of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture and Technology, Bhubaneswar - 751 003, IN

Source

Current Science, Vol 116, No 12 (2019), Pagination: 1955-1957

Abstract

No Abstract.

Keywords

No Keywords.

Full Text

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Techniques in Molecular Biology and Plant Biotechnology: A Compendium on Laboratory Experiments in Molecular Biology, Immuno-Diagnostics, Tissue Culture and Genetic Engineering in Plants. M. R. Shylaja, Deepu Mathew and K. V. Peter (eds)

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Current Science

Current Science

Author Details

Rout, G. R.

Establishing Taxonomic Identity and Selecting Genetically Diverse Populations for Conservation of Threatened Plants Using Molecular Markers

Authors

Source

Abstract

Keywords

Full Text

References

In vitro Propagation of Some Threatened Plant Species of India

Authors

Source

Abstract

Keywords

Full Text

References

A New Growth Medium for Identification and Isolation of Pyricularia grisea From Finger Millet (Eleusine coracana L. Gaertn.)

Authors

Source

Abstract

Keywords

Full Text

References

Techniques in Molecular Biology and Plant Biotechnology: A Compendium on Laboratory Experiments in Molecular Biology, Immuno-Diagnostics, Tissue Cul­ture and Genetic Engineering in Plants. M. R. Shylaja, Deepu Mathew and K. V. Peter (eds)

Authors

Source

Abstract

Full Text

Techniques in Molecular Biology and Plant Biotechnology: A Compendium on Laboratory Experiments in Molecular Biology, Immuno-Diagnostics, Tissue Culture and Genetic Engineering in Plants. M. R. Shylaja, Deepu Mathew and K. V. Peter (eds)